Digestive and Liver Disease - Volume 48 - Supplement 2 - February 2016

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Abstracts of the 22nd National Congress of Digestive Diseases / Digestive and Liver Disease 48S2 (2016) e67–e231 e161
Paired Sample T-Test and the analysis of covariance (ANCOVA) when
appropriate.
Results: In the treatment group, we observed a significant reduction
of situation and basic attitude anxiety (STAI-Y; t=-2.69 p=0.01
for the status anxiety and t=-3.87 p=0.0004 for trait anxiety), an
improvement in the ability to identify emotions (DIS subscale
of the TAS-20; t=-3.47 p=0.001) and to address the disease by
decreasing its denial (denial of stairs BRIEF-COPE t = -1.8 p=0.07).
Moreover, the psychological support provided evidence to improve
the management of less negative situation of the disease (positive
subscale restructuring BRIEF-COPE t=3.26 p=0.002) (subscale
emotional functioning IBDQ) which is reflected in a significant
increase in quality of life (measured with the IBDQ t=2.14 p=0.039)
and a decrease in the perception of negative emotions due to illness
(BRIEF-IPQ t=-1.72 p=0.09).
No significant change was found in control group. As far as quality of
life is concerned, IBDQ showed an increase in the treatment group,
significantly higher if comared to control group (7.91 vs -3.2) (t=2.14
p=0.039)
Conclusions: The psychological treatment proved to be successful
in reducing anxiety and depression as well as improving quality of
life in IBD patients. This kind of psychological support is easy to use,
even in the hospital setting, and can also be defined cheap.
P.07.10
FAECAL-ASSOCIATED AND MUCOSAL-ASSOCIATED MICROBIOTA
IN INFLAMMATORY BOWEL DISEASE PATIENTS AND HEALTHY
SUBJECTS: PRELIMINARY EVIDENCE
Guarino M.P.*2, Putignani L.3, Altomare A.2, Del Chierico F.4,
Cocca S.2, Tripiciano C.1, Dallapiccola B.3, Cicala M.2
1Campus Bio-Medico, Rome, Italy, 2Campus Bio-Medico University,
Gastroenterology Unit, Rome, Italy, 3Bambino Gesù Children’s Hospital
and Research Institute, Parasitology Unit, Metagenomics Unit, Rome,
Italy, 4Bambino Gesù Children’s Hospital and Research Institute,
Metagenomics Unit, Rome, Italy
Background and aim: Over the last few years, growing evidence
has supported the potential role of intestinal microbiota in the
pathophysiology and symptom generation of several gastrointestinal
(GI) diseases, such as Inflammatory Bowel Disease (IBD). The existing
literature on the intestinal microbiota in IBD does not reveal uniform
alterations in microbiota composition among all patients. Several
studies have seen abnormal GI microbiotas; however, the relevance
of such studies has been hampered by the fact that the analyses were
focused only on the faecal microbiota, which differs substantially
from that adhering to the gut mucosa. In the present study, we
evaluated the microbiota composition in intestinal mucosal biopsies
and faecal samples of IBD patients and control subjects (CTRLs) in a
case-control study exploited by 16S rRNA targeted metagenomics-
based approach (phylotyping, PH).
Material and methods: Faecal specimens were collected from 12
IBD patients and from 11 healthy subjects, undergone to colonoscopy
for screening. Colonic mucosal specimens were obtained during
colonoscopy from the proximal descending colon. All patients filled
out a standardized questionnaire for the GI symptoms and the
quality of life. All the patients were enrolled only after signing the
informed consent. PH was assessed by pyrosequencing as follows.
Genomic DNA was isolated from the entire set of samples using the
QIAamp DNA Stool Mini Kit (Qiagen, Germany). The V1-V3 region of
16S rRNA locus was amplified on a 454-Junior Genome Sequencer
(Roche 454 Life Sciences, Branford, USA). Reads were analyzed by
Quantitative Insights into Microbial Ecology (QIIME, v.1.8.0), grouped
into operational taxonomic units (OTUs) at a sequence similarity
level of 97% by PyNAST for taxonomic assignment, and aligned by
UCLUST for OTUs matching against Greengenes database (v. 13.8).
Results: In adult IBD patients colonic biopsies showed a statistically
significant increase of Proteobacteria and decrease of Firmicutes,
compared to CTRLs, with main OTUs in IBDs being Enterobacteriacee
and Clostridiales compared to Ruminococcacee, Prevotella,
Bacteroides and Faecalibacterium praustinizii in CTRLs (p<0.05). The
analysis of IBD faecal samples reproduced these data except for a
significant reduction of Clostridiales in IBD faecal samples, compare
to CTRLs.
Conclusions: Our data suggest the potential of microbiota profiling
in the descritption of disease-related microbiota enterogradients.
P.07.11
ASSOCIATION BETWEEN INFLAMMATORY BOWEL DISEASE AND
VITAMIN D DEFICIENCY: A SYSTEMATIC REVIEW AND META-
ANALYSIS
Del Pinto R.*1, Pietropaoli D.2, Chandar A.K.3, Ferri C.1, Cominelli F.3
1University of L’Aquila, Department of Life, Health and Environmental
Sciences, Division of Internal Medicine, St. Salvatore Hospital,
L’Aquila, Italy, 2University of L’Aquila, Department of Life, Health and
Environmental Sciences, Division of Dentistry, St. Salvatore Hospital,
L’Aquila, Italy, 3Case Western Reserve University, Department of
Gastroenterology, UH Hospitals, Cleveland, United States
Background and aim: Vitamin D plays a role in several immune-
mediated diseases, but its association with inflammatory bowel
disease (IBD) is unclear. We conducted a systematic review and
meta-analysis to assess the association between IBD and vitamin D
deficiency.
Material and methods: We searched electronic databases
from inception to December 2014 for observational studies
reporting the presence of vitamin D deficiency (defined as serum
25-hydroxycholecalciferol [25(OH)D] level of ≤20 ng/ml) in IBD
patients and having a control group without IBD. Odds ratios (OR)
were combined using a random effects model. Meta-regression was
performed using latitude as a moderator. Study quality was assessed
using the Newcastle-Ottawa scale.
Results: Out of 816 citations, 14 eligible studies were identified,
comprising 1891 participants (938 IBD cases and 953 controls).
Meta-analysis showed that patients with IBD had 64% higher odds
of vitamin D deficiency when compared to controls (OR=1.64;
95% CI: 1.30, 2.08; I2 = 7%; p < 0.0001). UC patients had more than
double the odds of vitamin D deficiency when compared to normal
controls (OR=2.28; CI: 1.18, 4.41; I2 = 41%; p=0.01). Meta-analysis of
the 3 studies reporting on children showed that 177 pediatric cases
with IBD had a higher, although not significant, odds of vitamin D
deficiency compared with 413 non-IBD controls (OR = 1.36; 95%; CI:
0.91, 2.04; I2=18%; p=0.14). Latitude did not influence the association
between IBD and vitamin D deficiency (p = 0.34). All studies were of
moderate to high quality as assessed by the Newcastle–Ottawa scale.
Generalizability of our results might be limited as we summarized
unadjusted ORs, due to non-availability of adjusted ORs in individual
studies.
Conclusions: IBD is significantly associated with having higher
odds of vitamin D deficiency. Well-designed RCTs and longitudinal
studies are needed to further clarify the role of vitamin D in IBD
pathogenesis and its therapy.