Digestive and Liver Disease - Volume 48 - Supplement 2 - February 2016

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e176 Abstracts of the 22nd National Congress of Digestive Diseases / Digestive and Liver Disease 48S2 (2016) e67–e231
Background and aim: Current knowledge on fecal microbiota and
fecal metabolome in Symptomatic Uncomplicated Diverticular
Disease (SUDD) of the colon is still lacking. We performed a
prospective study assessing fecal microbiota and metabolome in
those patients.
Material and methods: Stool samples from 52 consecutive female
patients (17 with SUDD, 16 with asymptomatic diverticulosis, and
19 healthy) were analysed. Real-time PCR was used to quantify
targeted microorganisms. High-resolution proton nuclear magnetic
resonance spectroscopy in combination with analysis of variance-
Simultaneous Component Analysis model were assessed in
determining fecal metabolome.
Results: The overall fecal bacterial quantity did not differ among
the three groups (p=0.449). The quantitative analysis of bacterial
populations showed no difference in the numbers of rRNA gene
copies neither for the total bacteria nor for the different types
analysed in the three study groups (Akkermansia: p=0.298;
Bacterioides: p=0.354; Bifidobacterium: p=0.876; Clostridium:
p=0.463; Escherichia: p=0.728; Lactobacillus: p=0.633). Overall, fecal
metabolome analysis was not able to detect any significant model.
However, methanol, glycine, U1 and U2 was found significantly
increased in SUDD.
Conclusions: SUDD does not show any significant quantitative and
qualitative alteration of the analysed fecal microbiota. However,
increasing expression of some metabolites as expression of different
SUDD metabolic activity were found.
P.09.13
ADVANCED ADENOMA IN SCREENING POPULATION AND LIFE
STYLE HABITS: A CASE CONTROL STUDY
Urban F.*, Giudici F., Tonello C., Paolini A., Simeth C., Consalvo D.,
Fabro M., Stefani N., Sozzi M., Zanconati F., Monica F.
Azienda Ospedaliero Universitaria “Ospedali Riuniti”, Trieste, Italy
Background and aim: Colorectal cancer (CRC) is the most frequent
tumor in Italy. Friuli Venezia Giulia Region has higher CRC incidence
than in population of Northern Italy (33 vs 21/100,000 men and
21 vs 12.1/100.00 females). Several risk factors may increase the
chance of developing CRC. Aim of this study was to evaluate if life-
style habits are linked to the development of advanced adenoma in
screening population in Trieste area.
Material and methods: In the present single centre, case-control
observational study, 50-74 years old asymptomatic subjects
participating in an organized CRC screening program based on
immunologic fecal occult blood test and undergoing colonoscopy
for positive test received a structured questionnaire investigating
lifestyle habits. Patients with advanced adenoma were matched on
age and sex with patients with negative colonoscopy (controls).
Results: Since January to May 2015, 400 pts were recruited and
data on 234 subjects (78 cases and 156 controls) were evaluated.
Seven variables were selected for multivariate logistic regression
analysis: daily intake of fresh fruit, daily 30’walking, units of alcohol
consumption, daily intake of raw vegetables, smoking habits, low
dose aspirin and vitamins per day. A protective role for the onset for
advanced adenoma was found for the intake of fresh fruit (OR for ≥2
vs. 0-1 portions 0.28 CI 0.08-0.78 p=0.02) and daily walking (OR 30’
or more walking vs. <30’ 0.42 CI 0.20-0.87 p=0.02).
Conclusions: Daily intake of fresh fruit and regular physical activity
are the only significant protective variables towards the onset of
colonic precancerous lesions. The results of this preliminary study
underline the importance of the implementation of educational
interventions for the whole population.
P.09.14
ROLE OF METABOLIC, ATHEROGENIC AND PSYCHOLOGICAL
FACTORS IN PATIENTS WITH COLORECTAL ADENOMAS
Boarino V.*1, Merighi A.1, Mancini S.2, Bertani A.1, Marocchi M.1,
Marsico M.1, Olivetti G.1, Primerano A.M.1, Mattei G.2, Ferrari S.2,
Roncucci L.2, Villa E.1
1 Gastroenterologia ed Endoscopia Digestiva A.O.U. Policlinico, Modena,
Italy, 2Dipartimento di Medicina Diagnostica, Clinica e di Sanità
Pubblica A.O.U. Policlinico, Modena, Italy
Background and aim: Proinflammatory states of large bowel have
a multifactorial aetiology, including metabolism, atherogenesis and
also psychological determinants. Inflammation is also a key factor
for the development of adenoma and colorectal cancer. The aim of
the study is investigate the association between pro-atherogenetic
factors, metabolic status, psychological assessment and the presence
of colorectal adenomas.
Material and methods: In our case-control observational ongoing
study, patients underwent colonoscopy for positive faecal blood
test and/or abdominal symptoms with negative history for
neoplasia or inflammatory bowel diseases. We collected waist and
hip circumferences, BMI, arterial pressure, fasten serum glycemia,
medical and drug history. Patients were analyzed, in blind for the
outcome of colonoscopy (adenoma vs no-adenoma), evaluating
carotid IMT (QIMT®-Esaote software) and psychometric assessment
of anxiety and depression using HADS (Hospital Anxiety and
Depression Scale).
Results: From January 2015 we analyzed 18 patients (8 M, 10 F),
mean age 62.6 years (range 48-77). 10 patients (M/F 6/4) had at
least one adenoma at colonoscopy, 8 (M/F 2/6) had no lesions. Data
analysis showed no significant difference between adenoma and
no-adenoma group for fasten serum glycemia, arterial pressure,
HADS score for anxiety, BMI and waist/hip ratio values. Significant
difference between adenoma vs no-adenoma group was found
for waist circumference and body weight (p=0.009 and p=0.03
respectively), carotid QIMT median value (p=0.037) and HADS
score for depression (p=0.026). We observed also an inverse
correlation between age and HADS anxiety score (p<0.05) and a
direct correlation between higher values fasten serum glycemia and
carotid QIMT (p<0.05).
Conclusions: Despite the small sample size and the preliminary
nature of data, the association between waist circumference, body
weight, higher values at the QIMT examination and presence of
adenomas confirms evidence of literature showing connections
between metabolism and risk of development of colorectal cancer.
Depression, and not anxiety, seems to be a factor characterizing
patients with adenomas versus adenoma-free group. In conclusion
our study provides interesting insights into the complex
relation between proinflammatory state, evaluated through a
multidisciplinary approach, and development of precancerous
colorectal lesions.
P.09.15
INCREASED ABUNDANCE OF BENEFICIAL BACTERIA IS
ASSOCIATED WITH CLINICAL IMPROVEMENT AFTER RIFAXIMIN
TREATMENT
Ponzani F.R.*1, Scaldaferri F.1, Pecere S. 1, Paroni Sterbini F.2, Petito V.1,
Lopetuso L.R.1, Gerardi V.1, Palladini A. 2, Graziani C.1, Masucci L.2,
Pompili M.2, Sanguinetti M.2, Gasbarrini A.1
1 Internal Medicine and Gastroenterology, A Gemelli Hospital, Rome,
Italy, 2Microbiology, A Gemelli Hospital, Rome, Italy
Background and aim: Rifaximin has been demonstrated to produce
a favorable modulation of the gut microbiota. However, whether
this beneficial effect is associated with clinical improvement is